Purpose:
To develop (or import the design of) a system that allows fast 3-D random access to spots in a specimen using multiphoton microscopy.This technology allows rapid access neurons located in different parts of the brain and achieve high temporal resolution imaging because no frame-by-frame scanning is required.
Several studies have achieve success in doing to by implementing acousto-optical deflectors. In some implementation, the AOD can be placed in the optical path outside of the scan head to make it easily adaptable onto different microscopes. One implementation (unpublished) is done in Institute of Neuroscience in Shanghai. I can help obtaining additional information.
I have placed several references in the U drive: U:\cry\wiw\AOD_random access\references. A list of references is below.
1: Ranganathan GN, Koester HJ. Optical recording of neuronal spiking activity
from unbiased populations of neurons with high spike detection efficiency and
high temporal precision. J Neurophysiol. 2010 Jul 7. [Epub ahead of print] PubMed
PMID: 20610791.
2: Grewe BF, Langer D, Kasper H, Kampa BM, Helmchen F. High-speed in vivo calcium
imaging reveals neuronal network activity with near-millisecond precision. Nat
Methods. 2010 May;7(5):399-405. Epub 2010 Apr 18. PubMed PMID: 20400966.
3: Duemani Reddy G, Kelleher K, Fink R, Saggau P. Three-dimensional random access
multiphoton microscopy for functional imaging of neuronal activity. Nat Neurosci.
2008 Jun;11(6):713-20. Epub 2008 Apr 27. PubMed PMID: 18432198; PubMed Central
PMCID: PMC2747788.
4: Iyer V, Hoogland TM, Saggau P. Fast functional imaging of single neurons using
random-access multiphoton (RAMP) microscopy. J Neurophysiol. 2006
Jan;95(1):535-45. Epub 2005 Oct 12. PubMed PMID: 16221746.
5. Kirkby PA, Srinivas Nadella KM, Silver RA.A compact Acousto-Optic Lens for 2D and 3D femtosecond based 2-photon microscopy.
Opt Express. 2010 Jun 21;18(13):13721-45. doi: 10.1364/OE.18.013720.
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